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Primer3 was a complete re-implementation of an earlier program: Primer 0.5 (Steve Lincoln, Mark Daly, and Eric S. Lander). Primer3 web service at primer3.ut.ee is a part of services provided by ELIXIR - European research infrastructure for biological information.
Primer3Plus picks primers from a DNA sequence using Primer3. This is the latest version straight from the developers with all the new features.
Primer3 is a program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers.
Primer3 was a complete re-implementation of an earlier program: Primer 0.5 ( Steve Lincoln, Mark Daly, and Eric S. Lander ). Lincoln Stein championed the idea of making Primer3 a software component suitable for high-throughput primer design. Web interface by Steve Rozen.
Pick right primer or use right primer. below (5'->3' on opposite strand).
Primer3 picks primers for PCR reactions, considering as criteria: o oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities, o PCR product size, o positional constraints within the source (template) sequence, and o possibilities for ectopic priming (amplifying the wrong sequence) o many other constraints.
Primer3Plus is an updated, task-oriented web-interface to Primer3. Primer3Plus is developed and maintained by Andreas Untergasser. Primer3Plus at www.primer3plus.com