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The Greene Lab. Columbia University. Department of Biochemistry and Molecular Biophysics. Our group uses single-molecule optical microscopy to study fundamental interactions between proteins and nucleic acids - we literally watch individual protein molecules or protein complexes as they interact with their DNA substrates.
Grammy award-winning American baritone Eric Greene’s current and future engagements include BENNY “KID” PARET in Terence Blanchard’s Champion Metropolitan Opera, ESCAMILLO Carmen Liceu Barcelona, return to London as NICHOLAS LOFTE in Itch, a new opera by Jonathan Dove, as well as join the Royal Opera House, Covent Garden for Rigoletto ...
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Bloom helicase mediates formation of large single-stranded DNA loops during DNA end processing. Xue C, Salunkhe SJ, Tomimatsu N, Kawale AS, Kwon Y, Burma S, Sung P, Greene EC. Nat Commun. 2022 Apr 26;13(1):2248. doi: 10.1038/s41467-022-29937-7. PMID: 35473934 Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments. Meir A, C...
Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks. Kong M, Greene EC. Front Cell Dev Biol. 2021 Nov 15;9:745311. doi: 10.3389/fcell.2021.745311. eCollection 2021. PMID: 34869333 Single-molecule studies of yeast Rad51 paralogs. Roy U, Kwon Y, Sung P, Greene EC. Methods Enzymol. 2021;661:343-362. doi: 10.1016/bs.mie....
Demystifying the D-loop during DNA recombination. Roy U,Greene EC. Nature. 2020 Oct 14. doi: 10.1038/d41586-020-02831-2. Online ahead of print. PMID: 33057188 Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex. Crickard JB, Kwon Y, Sung P,Greene EC. EMBO J. 2020 Oct 15;39(20):e105705. doi: 10...
Rad52 Restrains Resection at DNA Double-Strand Break Ends in Yeast. Yan Z, Xue C, Kumar S, Crickard JB, Yu Y, Wang W, Pham N, Li Y, Niu H, Sung P, Greene EC, Ira G. Mol Cell. 2019 Dec 5;76(5):699-711.e6. doi: 10.1016/j.molcel.2019.08.017. Epub 2019 Sep 18. PMID: 31542296 Structures and single-molecule analysis of bacterial motor nuclease AdnAB illu...
The biochemistry of early meiotic recombination intermediates. Crickard JB, Greene EC. Cell Cycle. 2018;17(23):2520-2530. doi: 10.1080/15384101.2018.1553355. Epub 2018 Dec 10. PMID: 30482074 New roles for RAD52 in DNA repair. Xue C, Greene EC. Cell Res. 2018 Dec;28(12):1127-1128. doi: 10.1038/s41422-018-0105-8. No abstract available. PMID: 30367126...
Dissociation of Rad51 Presynaptic Complexes and Heteroduplex DNA Joints by Tandem Assemblies of Srs2. Kaniecki K, De Tullio L, Gibb B, Kwon Y, Sung P, Greene EC. Cell Rep. 2017 Dec 12;21(11):3166-3177. doi: 10.1016/j.celrep.2017.11.047. PMID: 29241544 The condensin complex is a mechanochemical motor that translocates along DNA. Terakawa T, Bisht S,...
A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling. Taylor MRG, Špírek M, Jian Ma C, Carzaniga R, Takaki T, Collinson LM, Greene EC, Krejci L, Boulton SJ. Mol Cell. 2016 Dec 1;64(5):926-939. doi: 10.1016/j.molcel.2016.10.020. Epub 2016 Nov 17. PMID: 27867009 ATP hydrolysis Promotes Duplex DNA Releas...
Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System. Redding S, Sternberg SH, Marshall M, Gibb B, Bhat P, Guegler CK, Wiedenheft B, Doudna JA, Greene EC. Cell. 2015 Nov 5;163(4):854-65. doi: 10.1016/j.cell.2015.10.003. PMID: 26522594 DNA RECOMBINATION. Base triplet stepping by the Rad51/RecA family of recombinases. ...
Protein dynamics during presynaptic-complex assembly on individual single-stranded DNA molecules. Gibb B, Ye LF, Kwon Y, Niu H, Sung P, Greene EC. Nat Struct Mol Biol. 2014 Oct;21(10):893-900. doi: 10.1038/nsmb.2886. Epub 2014 Sep 7. PMID: 25195049 DNA curtains: novel tools for imaging protein-nucleic acid interactions at the single-molecule level....
How do proteins locate specific targets in DNA? Redding S, Greene EC. Chem Phys Lett. 2013 May 10;570. doi: 10.1016/j.cplett.2013.03.035. PMID: 24187380 Tension modulates actin filament polymerization mediated by formin and profilin. Courtemanche N, Lee JY, Pollard TD, Greene EC. Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9752-7. doi: 10.1073/pn...
- (212) 342-2943
- ecg2108@cumc.columbia.edu
Overview. Eric Greene is a professor at Columbia University, New York, New York, USA, in the Department of Biochemistry and Molecular Biophysics. He received his Ph.D. in biochemistry from Texas A&M University, College Station, USA, while working for Dorothy Shippen and conducted postdoctoral research with Kiyoshi Mizuuchi at the National ...
2008. The condensin complex is a mechanochemical motor that translocates along DNA. T Terakawa, S Bisht, JM Eeftens, C Dekker, CH Haering, EC Greene. Science 358 (6363), 672-676. , 2017. 300. 2017. Dynamic basis for one-dimensional DNA scanning by the mismatch repair complex Msh2-Msh6.
Department of Biochemistry and Molecular Biophysics. Our group uses single-molecule optical microscopy to study fundamental interactions between proteins and nucleic acids - we literally watch individual protein molecules or protein complexes as they interact with their DNA substrates.
Eric Greene. Associate Professor of Religious Studies. 203-432-4857. Field of Interest: Asian Religions. Address: 320 York St, New Haven, CT 06511. Website. Curriculum Vitae: greene_cv.pdf. Office Hours: Thursdays 12:30 - 2:30. B.A., M.A., Ph.D., University of California, Berkeley.
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