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  1. Learn how to push the limits of your Golden Gate Assembly, and try one of our convenient kits (using BsmBI-v2 or BsaI-HFv2). The efficient and seamless assembly of DNA fragments, commonly referred to as Golden Gate Assembly (1,2), has its origins in 1996, when for the first time it was shown that multiple inserts could be assembled into a vector backbone using only the sequential (3) or ...

  2. Push the Limits of Golden Gate Assembly. 50+ fragment DNA assembly achievable with high efficiency and accuracy! With constant advances both in the development of new enzymes (e.g., PaqCI ®, BsaI-HF ® v2 and BsmBI-v2) and research on maximizing enzyme functionality (e.g., ligase fidelity), NEB has become the industry leader in pushing the ...

  3. 10 nov 2014 · A worker atop the Golden Gate Bridge span applies a fresh coat of paint in 1956. The bridge is named for the Golden Gate Strait, the entrance to the San Francisco Bay from the Pacific Ocean.

  4. 2 giorni fa · The mission of the District is to provide safe and reliable operation, maintenance and enhancement of the Golden Gate Bridge and to provide transportation services, as resources allow, for customers within the U.S. Highway 101 Golden Gate Corridor. The Golden Gate Bridge, Highway and Transportation District is a special district of the State of ...

  5. Golden Gate Highlands National Park was established in 1963 to protect the sandstone rocks were once shelters for the Bushmen. Guests can view many of their cave paintings that are well preserved. The park also boasts various rare and indigenous flowers that include the Arum Lily, Watsonias, Fire Lilies and Red-Hot Pokers.

  6. 25 set 2022 · Reset. Nestled in the rolling foothills of the Maluti Mountains of the north eastern Free State lies the Golden Gate Highlands National Park. Rejuvenation is on offer at Golden Gate Highlands National Park. Spend time in the majestic Maluti Mountains, hiking, horse riding, exploring caves and much more in their indescribable golden glow.

  7. Gardens, glades, quiet lakes—Golden Gate Park is the emerald heart of San Francisco, a classic city park where everyone, from first-time visitors to go-every-weekend locals, finds something amazing to see or do. The park’s cultural hub is in its northeast corner, surrounding a broad concourse featuring fountains and a band shell.

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