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2 giorni fa · Using Primer3 software (version 2.5.0) 31, we designed primers with specific parameters: a primer sequence length ranging from 17 to 32 bp, a Tm value between 60 and 64 °C (with an optimal value ...
3 giorni fa · - Use bioinformatics tools like Primer3, NCBI Primer-BLAST, or similar to design specific primers. - Aim for primers around 18-22 nucleotides in length, with a GC content of 40-60%. - Ensure the primers have similar melting temperatures (Tm) to optimize annealing conditions.
2 giorni fa · The long non-coding RNA Meg3 mediates imprinted gene expression during stem cell differentiation. Sabina Farhadova and others. Nucleic Acids Research, Volume 52, Issue 11, 24 June 2024, Pages 6183–6200, https://doi.org/10.1093/nar/gkae247. Abstracts.
22 ore fa · Phytophthora cinnamomi is a devastating soil-borne plant pathogen. The primary source of P. cinnamomi infection is the soil, where the pathogen can persist for long periods. Effective prevention and management of this pathogen in tree crops requires an early and reliable detection method. In this study, we developed a simple, fast, reliable, and sensitive method based on real-time quantitative ...
4 giorni fa · 1 INTRODUCTION. Liver cancer is extremely common and the third leading cause of cancer-related deaths worldwide. Many risk factors have been associated with hepatocellular carcinoma (HCC), a common form of primary liver cancer in adults. 1 In addition to non-alcoholic steatohepatitis (NASH), hepatitis B and C virus (HBV-HCV) infection, and medical or genetic conditions, personal life habits ...
2 giorni fa · Process like DNA sequencing (to determine the exact order of nucleotides in a DNA) and polymerase chain reaction (or PCR, used to amplify DNA sequences) require DNA primers whereas for natural DNA replication short sequences of RNA is used as primer. Usually the length of the primer is 18 to 24 nucleotides.
5 giorni fa · Background Soapberry (Sapindus mukorossi) is an economically important multifunctional tree species. Triterpenoid saponins have many functions in soapberry. However, the types of uridine diphosphate (UDP) glucosyltransferases (UGTs) involved in the synthesis of triterpenoid saponins in soapberry have not been clarified. Results In this study, 42 SmUGTs were identified in soapberry, which were ...
