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centromeric plasmid (pBB247) or into the chromosome (by the integrative vector pAB247). Activation by the constitutively expressed activator In the simple activation system, rtTA is expressed constitutively from the CMV promoter (pBB140) and activates the expression of the chromosomally integrated reporter construct in the presence of doxycycline
15 mag 2001 · In order to detect the activator directly, we constructed a second class of autocatalytic circuits: the intrinsic reporter systems pBB247 and pAB247. GFP was inserted into rtTA, generating the fusion protein rtTA–GFP.
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- How It Works
- The Big Brother House
- Seasons and editions
- Presenters
- Theme Songs
- Social Media
- Trivia
Big Brotheris a reality television show in which a group of people lives together in a large house, isolated from the outside world but continuously watched by television cameras. Each series lasts for around three months, and there are usually fewer than 15 participants. The housemates try to win a cash prize by avoiding periodic evictions from th...
For more information about the house interiors, check out the page Big Brother House/Philippines. The Big Brother house, commonly called in Filipino as "Bahay ni Kuya" (lit. Big Brother's House), plays a huge part in the show. The reality series' house is famous for its yellow and blue facade designed to look like a typical two-story bungalow house...
Since the show began airing in 2005, Pinoy Big Brother has successfully produced 8 regular seasons (4 civilian; 4 special), 4 teen seasons, and 2 celebrity seasons.
Currently, Pinoy Big Brother is spearheaded by main presenter, Bianca Gonzalez, along with co-hosts Robi Domingo, Melisa Cantiveros, Kim Chiu, Enchong Dee, and Richard Juan. From its inaugural season in 2005 until 2022, actress, Singer, and TV Host Toni Gonzaga served as the show's main presenter, and was an integral part of the show. However, in 2...
The series has produced three different theme songs specifically used on every type of edition aired.
Pinoy Big Brother is the longest-running Big Brother series in Asia, followed by India's Bigg Boss.Pinoy Big Brother is the only Big Brother franchise in the world to adopt a Teen Edition for Big Brother after Teen Big Brother: The Experimentwas aired in the United Kingdom in 2003.Since its launch in 2005, Pinoy Big Brother already had over 300 housemates living inside the house.All of the show's current presenters experienced living inside the house.5 lug 2015 · Jul 04, 2015, 8:01 pm. ABS-CBN management has ended the all-day free television and online streaming of reality program "Pinoy Big Brother (PBB) 737" after the show and its housemates were subjected to malicious comments online. In an official statement emailed to Sun.Star, the network announced that they are cutting off the 24/7 live streaming ...
27 lug 2019 · FULL EPISODE: PBB Otso Weekend | July 27, 2019 | #PBB8BigOtso | For more exclusive updates follow us on –– FB and IG: @pbbabscbntv Twitter: @pbbabscbn...
