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Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright's stain, Giemsa stain, or Diff-Quik. Wright-Giemsa combination stain is also a popular choice. These stains allow for the detection of white blood cell, red blood cell, and platelet abnormalities.
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8 ott 2020 · Staining of blood smear. Preparation of blood smear. 1) Thin Blood Film. Thin PBF can be prepared from anticoagulated blood obtained by venipuncture or from free-flowing finger-prick blood by any of the following three techniques: 1. Slide method. 2. Cover glass method. 3. Spin method. Slide Method. Procedure.
The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. This article highlights the basic science and art behind the PBF.
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Blood film preparation methodologies include (1) manual (wedge or coverslip); (2) semiautomated (wedge or spinner); and (3) automated (wedge). Typically, for wedge film preparations, a small amount of blood is placed on a glass slide, which is then spread (by a hand-held spreader slide).
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Examination of Blood Films. Stained blood films may be examined with or without a coverslip. When a coverslip is not used, a drop of immersion oil can be placed on the stained smear to reduce diffraction and facilitate microscopic examination at low power and with the high-dry objectives.