Risultati di ricerca
28 mar 2023 · Here we show that RfxCas13d, also known as CasRx, a widely used Cas13 system, can cause collateral transcriptome destruction when targeting abundant reporter RNA and endogenous RNAs, resulting in...
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11 ago 2022 · Several engineered Cas13 variants achieve targeted RNA degradation with minimal collateral effects. CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various organisms.
Collateral FX
3 gen 2024 · In molti casi, è possibile prevenire o mitigare gli effetti collaterali attraverso una valutazione accurata del paziente, una scelta oculata dei farmaci e una stretta supervisione medica durante il trattamento.
12 ago 2022 · This paper reports collateral effects of the CRISPR–Cas13a system in eukaryotes for the first time and demonstrates the powerful tumor-eliminating potential of this system.
1 feb 2023 · Here, we find that knocking down gene expression using RfxCas13d in the adult brain neurons caused death of mice, which may result from the collateral activity of RfxCas13d rather than the loss of target gene function or off-target effects.
Here, we design a dual-fluorescence reporter system for detecting collateral effects and screening Cas13 variants in mammalian cells. Among over 200 engineered variants, several Cas13 variants including Cas13d and Cas13X exhibit efficient on-target activity but markedly reduced collateral activity.
