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Primer3 was a complete re-implementation of an earlier program: Primer 0.5 ( ). Primer3 web service at primer3.ut.ee is a part of services provided by ELIXIR - European research infrastructure for biological information. For other services provided by ELIXIR's Estonian Node visit https://elixir.ut.ee/services . (input.htm v 4.1.0)
Primer3. Primer3 is a program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers. PCR is used for many different goals.
Primer3Plus picks primers from a DNA sequence using Primer3. This is the latest version straight from the developers with all the new features.
Primer3 was a complete re-implementation of an earlier program: Primer 0.5 ( Steve Lincoln, Mark Daly, and Eric S. Lander ). Lincoln Stein championed the idea of making Primer3 a software component suitable for high-throughput primer design. Web interface by Steve Rozen.
Pick hybridization probe. (internal oligo) or use oligo below. Pick right primer or use right primer. below (5'->3' on opposite strand).
Primer3 is a free online tool for designing PCR primers and probes. It can handle various types of input and output formats, and offers many options and parameters.
Primer3 picks primers for PCR reactions, considering as criteria: o oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities, o PCR product size, o positional constraints within the source (template) sequence, and o possibilities for ectopic priming (amplifying the wrong sequence) o many other constraints.
