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  1. Primer3 was a complete re-implementation of an earlier program: Primer 0.5 (Steve Lincoln, Mark Daly, and Eric S. Lander). Primer3 web service at primer3.ut.ee is a part of services provided by ELIXIR - European research infrastructure for biological information.

    • Available Species

      Primer3 k-mer lists. The files ending with 11 and 16 are...

    • Primer Task

      Primer3 favors product-size ranges to the left side of the...

    • Version 0.4.0

      Primer3 was a complete re-implementation of an earlier...

  2. primer3.orgPrimer3

    Primer3 is a program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers.

  3. Primer3Plus picks primers from a DNA sequence using Primer3. This is the latest version straight from the developers with all the new features.

  4. Pick hybridization probe. (internal oligo) or use oligo below. Pick right primer or use right primer. below (5'->3' on opposite strand).

  5. Primer3 is a free online tool for designing PCR primers and probes. It can handle various types of input and output formats, and offers many options and parameters.

  6. Primer3 picks primers for PCR reactions, considering as criteria: o oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities, o PCR product size, o positional constraints within the source (template) sequence, and o possibilities for ectopic priming (amplifying the wrong sequence) o many other constraints.

  1. Ricerche correlate a "primer3"

    reverse complement
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